Figure 1. Typical size-exclusion HPLC elution profiles of the water-soluble proteins from selected layers of porcine lens.  The layers are numbered consecutively from the lens periphery into the center.  Numbers above the chromatogram indicate positions at which various molecular weight standards eluted.  From lens periphery to center, HPLC profiles show a decrease in HMW proteins, an increase in LMW proteins and a change in proportion of the MMW proteins.