Figure 7. The effect of MMC on TGFBIp protein expression. Cells were incubated with 0.02% MMC for 6 h after which samples of media were taken, and the cells harvested. Cell lysates (15 μg) and media (30 μg) were analyzed for TGFBIp protein levels using immunoblotting. MMC reduced TGFBIp levels in both cell lysates and media for all 3 cell types. MMC: mitomycin C, NR: normal, HE: heterozygote, HO: homozygote.