Figure 6. The effect of MMC on Bcl-xL, Bax, and TGFBI mRNA expression in corneal fibroblasts. Cells were incubated with 0.02% MMC for 6 h after which RNA was isolated and subjected to RT–PCR analysis to determine the levels of Bcl-xL, Bax, and TGFBI mRNA expression. MMC reduced Bcl-xL mRNA expression in heterozygote (p=0.045) and homozygote (p=0.017) cells and markedly enhanced Bax expression in GCD II homozygote cells (p<0.01). MMC decreased TGFBI mRNA expression statistically significant in homozygote cells (p=0.025). The asterisk indicates a p<0.05; ANOVA compared with normal cell. MMC: mitomycin C, NR: normal, HE: heterozygote, HO: homozygote.