Figure 5 of Kim, Mol Vis 2008; 14:1222-1228.


Figure 5. The effect of MMC on corneal fibroblast apoptosis. Cells were incubated with 0.02% MMC for 6 h after which they were stained with annexin V then avidin-horseradish peroxidase (HRP) complex (1:300), and binding was visualized using a 0.05% diaminobenzidine/0.01% H2O2 solution. Cells were photographed using a fluorescence microscope equipped with an annexin V filter. Following MMC incubation, the GCD II homozygote cells showed cytoplasmic shrinkage and perimembranous stippling prominently compared with normal cells. MMC: mitomycin C, NR: normal, HE: heterozygote, HO: homozygote.