Appendix 5 of Clement, Mol Vis 2008; 14:1209-1221.


Appendix 5. Rabbit HSV-1 Latent trigeminal ganglia gene expression array means, their fold-changes with standard deviations and corresponding quantitative real-time polymerase chain reaction results of six selected genes

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Change in gene expression of six selected genes in HSV-1 latent rabbit trigeminal ganglia were compared to naive (control) based on microarrays (Array) and quantitative real-time polymerase chain reaction. The mean log2 difference and standard error of mean log2 difference (Microarray value) for each of the selected genes is logarithmic and was obtained by the averaging the difference between microarray values for infected (L) and microarray values for naive (N) of each gene before conversion. Listed are the corresponding processed red and green signal values from the microarrays. The fold change in gene expression (Array and Quantitative real-time polymerase chain reaction) is arithmetic. Relative quantitative expression levels were determined for each gene. All results are expressed as an expression ratio of the HSV-1 latent trigeminal ganglia to naive trigeminal ganglia, normalized against housekeeping GAPDH expression levels using the 2-ΔΔCT method [29]. All signal values of the arrays are distinguishable from background values, and most are high. The raw red and green signal values for the approximately 3,000 genes, 55,000 values are available on the Interdisciplinary Center for Biotechnology Research website.