Table 1 of Ponnam, Mol Vis 2008; 14:1204-1208.

Table 1. Details of polymerase chain reaction primers for LIM2 amplification.

Exon Primer name Primer sequence (5′-3′) Product size (bp) Annealing Temp (°C)
I LIM 1F CCATTGTGTAGGGAGGCTTA 213 52

LIM 1R AGGTCCTGGGAGAAGAAGG


II LIM 2AF CAGTTCCTCCCTTCAAGTCC 159 53

LIM 2AR ACTGCATCCAGTGGTCTGTT


LIM 2BF TGTACAGCTTCATGGGTGGT 255 52

LIM 2BR TGGAATACAGGTGTCCTTGG


LIM 2CF TACCTGCAGACAGACAGCAT 238 52

LIM 2CR CCCAACTTAACCTTCAAACC


III LIM 3F TCATCTCAGAGGTAGCAGCA 279 52

LIM 3R ATTGGGGTTTGAGATGAGAG


IV LIM 4F AAAATCACACCCAGCCTTAG 248 51

LIM 4R ACTCTATCTGCTGCCCACTC


V LIM 5F GGTGTTGGGCTCTCTTG 231 51

LIM 5R CTAGGAACCAGGATTTCA

Sequences of forward (F) and reverse (R) primers used for amplification of LIM2 exons are shown above along with sizes of PCR products for each primer pair. Exon 2 was amplified in three fragments using primer sets denoted 2A-2C.

Ponnam, Mol Vis 2008; 14:1204-1208. http://www.molvis.org/molvis/v14/a141

©2008 Molecular Vision http://www.molvis.org/molvis/

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