Figure 9. LSCM images of triple-labeling for actin, pan-cadherin and myosin IIA in the peri-sutural region. A: DIC image demonstrating the location of the beveled edge of the Vibratome section; the lower right arrow indicates the edge of the section and the upper left arrow indicates the extent of the bevel. B: Actin immuno-fluorescence (red) delineates the fiber end profiles, which are typically heterogeneous in size and shape in this region. C: Merged actin and pan-cadherin (blue) fluorescence demonstrates nearly complete colocalization in the BMC (purple). D: Merged actin and myosin IIA fluorescence (green) shows the diffuse distribution of myosin. The areas where these components are colocalized appear orange, indicating that actin predominates at the BMC periphery. E: Merged myosin IIA and pan-cadherin shows a comparable pattern to that in D, i.e. although both proteins are colocalized at the borders of profiles, the deep turquoise color indicates that pan-cadherin fluorescence is more pronounced. F: Merged actin, pan-cadherin, and myosin IIA fluorescence demonstrates the expected distribution. The colocaliztion of all three components appears white to pink. A through F show the same field of view and are at identical magnification.