Figure 8. LSCM visualization of double-labeling for actin and pan-cadherin in the equatorial segments of elongating fibers. A: Flattened hexagonal fiber cross-sections showed actin (red) concentrated along the short faces with faint label along broad faces. B: Cadherin (blue) was more prominent along the short sides of fiber profiles whereas the broad sides demonstrated reduced and often discontinuous labeling (arrows). C: As expected, merged actin and cadherin fluorescence (purple) displayed a high degree of colocalization. Fibers shown are between 95 and 115 cells deep to the equatorial capsule, corresponding to fiber ends in the distal portion of the lateral-posterior region of fiber end migration. A through C are at the same magnification.