Figure 6. LSCM images of double-labeling
for N-cadherin and F-actin at a posterior suture branch. Both
N-cadherin (A, inset in D) and F-actin (B) were
present at the margins of the BMC in the peri-sutural region. The
merged image (C) of N-cadherin (red) and F-actin (green)
fluorescence showed that they were largely co-localized at cell borders
(yellow to orange coloration). However, in the sutural region,
immunofluorescence for N-cadherin was conspicuously decreased (asterisk
in A and sutural region in C). The location of the
suture branch is indicated in the DIC image (D, arrows). A
through D are at identical magnification. Inset is a higher
magnification of boxed area in panel A (bar=10 µm) showing
N-cadherin labeling in the BMC and demonstrates that N-cadherin was
distributed fairly evenly around the BMC periphery.