Figure 2. LSCM visualization of BMC
components in whole-mount lens capsules at the equatorial region. A-B:
Paired fluorescent (A) and DIC (B) images of F-actin
labeled with phalloidin-FITC. F-actin is concentrated at cell borders. C-D:
Paired fluorescent (C) and DIC (D) images of (non-muscle)
myosin IIA labeling. Myosin is distributed throughout the BMC and
enriched at the cell periphery. E-F: Paired fluorescent (E)
and DIC (F) images of pan-cadherin labeling. As expected,
cadherin was localized to lateral membranes. G-H: Paired
fluorescent (G) and DIC (H) images of β1 integrin
labeling. Distribution of β1 integrin label was diffuse, forming a
plaque at the basal portion of the cells (asterisks). Micrographs A-H
are at equivalent magnifications.