Figure 10. LSCM images of double-labeling for β1 integrin and F-actin in the peri-sutural region. Whereas β1 integrin (A) was distributed throughout the BMC as a plaque, F-actin (B) was most prominent at the margins of fiber ends. A merged fluorescent image (C) of β1 integrin (green) and F-actin (red) showed that they were not markedly co-localized. White asterisks denote profiles without integrin label. D shows the paired DIC image of the same field of view. Fiber ends are discernable (black asterisks). A through D are at the same magnification.