Figure 1. Expression of the egr-1-d2EGFP
transgene in adult rat retina. Representative fluorescence microscopic
images of transverse sections of adult rat retina showing detection of
total cells (DAPI staining) and d2EGFP immunoreactivity (green
fluorescent protein, GFP). Animals were killed at 24:00 h. Panels A-C
show egr-1-d2EGFP transgenic rats and panels D-F show wild-type
rats. Note detection of abundant GFP immunoreactivity in the outer
nuclear layer (ONL), photoreceptor inner segment (IS), and
photoreceptor outer segment (OS) of the transgenic rat only. In
addition, single GFP-positive inner nuclear layer (INL; horizontal
arrowhead) and ganglion cell layer (GCL, vertical arrowhead) cells are
indicated in A. The scale bar represents 20 μm.