Figure 3. Immunofluorescence images on flat-mounted corneas in a model of endotoxin-induced uveitis analyzed with a 3D laser confocal microscope with a 20X enlargement. Immunostainings with anti-CD68 for macrophages (A), anti-TCR alpha/beta for lymphocytes (B), anti-MCA967 for granulocytes (C), and IgG1 mouse antibody for isotypic control (D) were revealed with secondary antibody, Alexa Fluor (in green) while the nuclear chromatin was stained with propidium iodide (in red). A double immunostaining with Alexa Fluor 488 phalloidin (in green), and antibody to CD68 (in red) was performed to localize the macrophages among the corneal layers, notably on the endothelium (E).