Figure 1 of Trinh, Mol Vis 2008; 14:1149-1156.


Figure 1. Correlation between in vivo confocal microscopy with immunohistochemistry on cornea in a model of Endotoxin Induced Uveitis. Heidelberg Retina Tomograph II (HRT II) in vivo confocal microscopy images of the cornea (400x400 μm) in a model of endotoxin-induced uveitis (A, C, E, and F) are correlated with ex vivo immunostaining images with Alexa Fluor 488 phalloidin staining cytoskeleton (in green) and counterstained with propidium iodide for nuclei (in red) on flat-mounted corneas that were analyzed by confocal microscopy of the same animals with a 20X enlargement (B and D). Inflammatory cells infiltrated the anterior stroma (A and B) and accumulated on the corneal endothelium, which appeared as a honeycomb-like mosaic (C and D). The anterior stroma (small arrow) and endothelium (large arrow) are shown on a corneal section by in vivo confocal microscopy (E). A clot of fibrin was individualized in the anterior chamber by HRT II (F).