Table 1 of Kane, Mol Vis 2008; 14:1138-1148.

Table 1. Limited cycle and real time PCR primers

Limited cycle PCR primers
Gene Sense primer 5′-3′ Antisense primer 5′-3′ Probe 5′-3′
VEGF CTGCTCTACCTCCACCATGC CTGCATTCACATTTGTTGTGC
CHL-1 TCTGTCACCTTTTGCAGTGG GTTGCCGATTCTGAA AGAGC
Cox2 AACAGGAGCATCCTGAATGG ATTTCATCTGCCTGCTCTGG
RTP801 TCTTAGCAGTTCTCGCTGACC TGGCACACAAGTGTTCATCC
BMP-2 CAAGATGAACACAGCTGG TTTGCTGTACTAGCGACACC
BMP-4 GGCTGTCAAGAATCATGG GCTCAGGATACTCAAGAC
18S GTGGAGCGATTTGTCTGGTT CGCTGAGCCAGTCAGTGTAG
Real time PCR primers
Gene Sense primer 5′-3′ Antisense primer 5′-3′ Probe 5′-3′
VEGF GTGCCCACTGAGGAGTCCA GTGCTGGCCTTGGTGAGGT CATCACCATGCAGATTATGCGGATCAA
Cox2 AGCCCTTCCTCCTGTGCCT CAGGAAGCTGCTTTTTACC TGATTGCCCGACTCCCTTG
CHL-1 CGTAGCTGAAGGGCTCTTT ACACGTTTCCCTCCGAACA AAATCGGCAACCCAATCAAT

Limited cycle PCR was performed using primers, designed using Primer3 software [11]. For real-time, PCR probes were labeled with 5′-FAM and 3′-TAMRA as quencher with the exception of the ribosomal probe, which was labeled with 5′-VIC to facilitate multiplexing.

Kane, Mol Vis 2008; 14:1138-1148. http://www.molvis.org/molvis/v14/a135

©2008 Molecular Vision http://www.molvis.org/molvis/

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