Figure 1. Validation of three genes
differentially expressed in human retinal pericytes (hRPC) in response
to hypoxia. The upregulation of a selection of genes was validated with
real time PCR, using a PerkinElmer 7700 analyzer, on cDNA generated
from human retinal pericytes exposed to increasing periods of hypoxia
(0, 6, 24, and 48 h). All results were normalized to 18S rRNA, using a
pre-developed assay reagent. Data are expressed as mean relative
quantity of mRNA, relative to control, for three independent
experiments ±standard error of measurement for (A) CHL-1
mRNA, (B) VEGF mRNA, and (C) Cox 2 mRNA.
Data are expressed as mean±SEM values. The asterisk indicates a
significance at p<0.05 and the double asterisk indicates a
significance at p<0.001.