Figure 3. TGFBI/BIGH3 expression
analyzed by Southern and western blots. A: The Southern blot
shows various integration sites of the transgene in 27 PGK-Groenouw F1
transgenic animals originating from four different founders. B:
The Western blot against TGFBI/BIGH3 of the wild type (left panel)
shows no expression in the retina and the brain in comparison with the
western blot of a PGK-Groenouw transgenic mouse (right panel). The
following abbreviations are used in (B): cornea (C ), retina (R
), brain (B), heart (H), liver (L), spleen (S), kidney (K), and muscle
(M). The arrow indicates 64 kDa band corresponding to keratoepithelin.