Figure 2. Screening for transgenic TGFBI/BIGH3 mRNA expression. A: The scheme shows the difference between human and murine TGFBI/BIGH3. Note the localization of the primers used for the PCR and the unique BamHI site present only in the human gene. B: Migration of undigested corneal (lanes 2 and 4) or BamHI digested retinal (lanes 1 and 3) cDNA amplimers from two different PGK-Groenouw transgenic mice, as showed in A. L indicates the 1-kb DNA ladder lane.