Figure 5. The effect of P58^IPK on gene expression of ATF4, C/EBP homologous protein, GRP78 and vascular endothelial growth factor on human retinal capillary endothelial cells by western blot. A: Evaluation the effect of P58IPK on gene expression of vascular endothelial growth factor (VEGF) on human retinal capillary endothelial cells (HRCECs) treated with tunicamycin by western blot. Normal represents normal control that was not endothelial reticulum (ER)-stressed; P58^IPK represents HRCECs transfected with rAAV2-P58^IPK and treated with tunicamycin ; ER stress represents HRCECs treated with tunicamycin; RNAi represents HRCECs transfected with pGIPZ-P58^IPK RNAi, and treated with tunicamycin. The expression level of VEGF in either group was: 0.06±0.01, 0.08±0.01, 0.13±0.02; and 0.17±0.02 (p<0.001), respectively. B: Evaluation the effect of P58^IPK on gene expression of ATF4 on HRCECs treated with tunicamycin by western blot: Normal represents normal control that was not ER-stressed; P58^IPK represents HRCECs transfected with rAAV2-P58^IPK and treated with tunicamycin ;ER stress represents HRCECs treated with tunicamycin;RNAi represents HRCECs transfected with pGIPZ-P58^IPK RNAi and treated with tunicamycin. The expression level of ATF4 in either group was: 0.12±0.03, 0.16±0.04, 0.25±0.03, and 0.33±0.04 (p<0.001), respectively. C: Evaluation the effect of P58^IPK on gene expression of C/EBP homologous protein (CHOP) on HRCECs treated with tunicamycin by western blot. Normal represents normal control that was not ER-stressed; P58^IPK represents HRCECs transfected with rAAV2-P58^IPK and treated with tunicamycin; ER stress represents HRCECs treated with tunicamycin; RNAi represents HRCECs transfected with pGIPZ-P58^IPK RNAi and treated with tunicamycin. The expression level of CHOP in either group was: 0.08±0.02, 0.10±0.03, 0.18±0.04, and 0.26±0.03 (p< 0.001), respectively. D: Evaluation the effect of P58^IPK on gene expression of GRP78 on HRCECs treated with tunicamycin by western blot. Normal represents normal control that was not ER-stressed; P58^IPK represents HRCECs transfected with rAAV2-P58^IPK and treated with tunicamycin; RNAi represents HRCECs transfected with pGIPZ-P58^IPK RNAi and treated with tunicamycin; ER stress represents HRCECs treated with tunicamycin. The expression level of GRP78 in either group was: 0.10±0.02, 0.12±0.03, 0.22±0.02, and 0.17±0.02 (p<0.001), respectively.