Figure 2. Effect of P58^IPK on apoptosis in human retinal capillary endothelial cells s after tunicamycin-induced endoplasmic reticulum stress. There was no significant difference between groups 1 (control) and 3 (rAAV2- P58^IPK). Apoptosis levels were higher than groups 1 and 3 in group2. Apoptosis levels were significantly higher in group 4 (co-transfected with pGIPZ- P58^IPK RNAi and rAAV2- P58^IPK) compared to all other groups (p<0.001 for all). Group 1 represents normal control that was not endoplasmic reticulum-stressed. Group 2 represents human retinal capillary endothelial cells (HRCECs) treated with tunicamycin. Group 3 represents HRCECs transfected with rAAV2-P58^IPK and treated with tunicamycin. Group 4 represents HRCECs transfected with pGIPZ-P58^IPK RNAi and treated with tunicamycin.