Figure 1. P58^IPK expression in human retinal capillary endothelial cells in different groups. A: P58IPK expression in human retinal capillary endothelial cells (HRCECs) transfected with rAAV2-GFP, pGIPZ-GFP, rAAV2- P58^IPK, and pGIPZ-P58^IPK RNAi as determined by semi-quantitative PCR. There were significant differences in the expression levels of P58^IPK/GAPDH among the four transfection groups (p<0.001). The highest level was in the group transfected with rAAV2- P58^IPK (0.63±0.04) while the lowest was in the group with pGIPZ- P58^IPK RNAi (0.23±0.04). No significant differences in P58^IPK/GAPDH levels were found between the rAAV2-GFP (0.35±0.05) and pGIPZ-GFP groups (0.41±0.04). B: P58^IPK expression in (HRCECs) transfected with rAAV2-GFP, pGIPZ-GFP, rAAV2- P58^IPK, and pGIPZ-P58^IPK RNAi as determined by real time PCR. There were significant differences in the expression levels of P58^IPK/GAPDH among the four transfection groups (p<0.001). The ratio of P58^IPK/GAPDH was 1.45±0.09 in the rAAV2- P58^IPK group and 0.41±0.06 in the pGIPZ- P58^IPK RNAi group. No significant differences in P58^IPK/GAPDH levels were found between the rAAV2-GFP (0.76±0.05) and pGIPZ-GFP groups (0.72±0.05).