Figure 2. Western blot analysis of NF-κB P65 protein levels in control and rd retinas for each age group. A: Little expression of NF-κB P65 (bands at 65 kDa) was found in the control retinas at different age groups. B: NF-κB P65 protein was detected in the samples from rd retinas. GAPDH (35 kDa) was used as a loading control. C: Intensitometry of immunoreactive bands in rd retinas. Note the protein levels of NF-κB P65 in rd retinas were significantly increased at P10 and reached a peak at P12. It kept a high level up to P18 and was markedly reduced by P28 (*p<0.05, compared with the control retina, n=6).