Figure 1. Immunochemical localization of NF-κBP65 in rd retinas and controls. A: In control retinas, weak immunoreactivity was present in the cytoplasm of cells in the ganglion cell layer (GCL), inner plexiform layer (IPL), and outer plexiform layer (OPL). The inserted picture showed magnified positive cells in the OPL. B: In the rd retina at P12, P65 immunoreactivity peaked, and prominent nuclear labeling of the cells was evident in the OPL. P65 immunoreactivity remained in the cytoplasm in the cells of GCL. The inserted picture in the upper right angle showed a magnified cell with nuclear labeling in the OPL. The inserted picture in the lower right angle showed a magnified cell with cytoplasm labeling in the GCL C: In the rd retina at P14, migration of the cells with intense nuclear labeling was seen toward the outer nuclear layer (ONL). The inserted picture showed a magnified cell with nuclear labeling in the ONL. D: In the rd retina at P16, P65 immunoreactivity was still prominent, but the cells with nuclear labeling were hardly seen. E-G: Double labeling of NF-κB P65 and CD 11b in the rd retina at P14 showed co-localization of NF-κB in microglial cells in the outer retina. E: Microglial cells were shown in green color; F: NF-κB P65 immunoreactivity was shown in red color. G: Expression of NF-κB P65 in the microglial cells was shown in orange color. The arrow shows positive labeling. In the figure, the inner nuclear layer is abbreviated INL. Scale bar equals 100 μm.