Figure 5. Epidermal adult stem cells can be converted into corneal epithelium by corneal stroma. A: PAS staining of the normal cornea epithelium showed no goblet cells, 200X. B: PAS staining of the reconstructed cornea epithelium also show no goblet cells, 200X. C-D: PAS staining shows the invaded goblet cells in the corneal surface of control group, 200X. The black arrow in C is pointing to the invaded goblet cells, the red arrow in D is pointing to the blood vessels. E: Normal cornea is shown, and the AB-PAS staining is red, characteristic of neutral mucus, 200X. F: The reconstructed cornea is shown, and the AB-PAS staining is also red, characteristic of neutral mucus, 200X. G: AB-PAS staining show goblet cells as purple, which is characteristic of mixed mucus, 200X. The red arrow in G is pointing to the goblet cells in the corneal epithelium of control group which were stained purple by the AB-PAS staining method. The results of immunohistochemistry showed that normal cornea epithelium (H) expressed CK3 (I.BB.787), 400X, as well as reconstructed cornea epithelium (I) expressed CK3, 400X. Both normal cornea epithelium (J) and reconstructed cornea epithelium (K) expressed CK12 (SC-17099) which is a specific marker for differentiated corneal epithelium, 400X. Furthermore, both of normal cornea epithelium (L) and reconstructed cornea epithelium (M) expressed PAX-6 (AB5409), 400X. Although the reconstructed epithelium of control group (N) expressed PAX-6 (AB5409), it did not express CK3 (O), showing that this kind of epithelium derived from the control group did not share the character with normal corneal epithelium, 200X. The scale bar represents 100 μm.