Figure 3. Human amniotic membrane serving as a niche for epidermal adult stem cells and the formation of EpiASC-HAM sheet. A: Passage 3 epidermal stem cell was used as seed cell to reconstruct artificial corneal epithelium, 100X. B: EpiASC cultured on HAM for 30 min, 100X. C: EpiASC cultured on HAM for 20 days and differentiated into keratinocytes expressing CK1/10 (ab20121), 100X. D: P63-positive EpiASC surrounded by P63-negative cells, showing EpiASC reconstructing a niche on HAM, 100X. E: P63-positive pattern of ESH, 200X. F: P63 (4A4) expression in a wavy pattern along the basal membrane of normal corneal limbus, 200X. The scale bar represents 100 μm.