Figure 3 of Agudo, Mol Vis 2008; 14:1050-1063.


Figure 3. Time course regulation of cell death–related proteins in naïve and optic nerve injured retinas. Western blot time course analyses showing the regulation of tumor necrosis factor receptor superfamily member 1a (TNFR1a), tumor necrosis factor receptor type 1, associated death domain (TRADD), total Caspase 3, active Caspase 3, and c-fos in naïve, intraorbital nerve transection (IONT)-, and intraorbital nerve crush (IONC)-injured retinas. Graphs show quantification of protein signals (n=4 animals per lesion and time point, western blots were replicated three times). The signal from injured retinas is referred to the naïve signal, which was arbitrarily considered 100%. To verify the amount of loaded protein, western blots were incubated with β-actin (an example is shown). Error bars show the standard error of the mean (SEM) for each experiment.