Figure 1. Time course and number of sequences regulated after optic nerve injury in the adult rat retina. A: Graph depicting the total number of sequences regulated in the retina specifically by intraorbital nerve transection (IONT; black columns), specifically by intraorbital nerve crush (IONC; white columns) and commonly by both lesions (gray columns) B: In this graph is shown the number of genes specifically up or down-regulated in the retina by IONT (black columns), specifically up or down-regulated by IONC (white columns) and commonly up or down-regulated by both lesions (gray columns). C: Graph on the left illustrates the time course and number of genes up or down-regulated in the retina at each time after IONT (black bars represent genes specifically regulated by transection and gray bars genes regulated by transection and crush). Graph on the right illustrates the time course and number of genes up or down-regulated in the retina at each time after IONC (white bars represent genes specifically regulated by crush and gray bars genes regulated by transection and crush). D: Graph on the left illustrates time course and number of expressed sequence tags (ESTs) up or down-regulated in the retina at each time after IONT (black bars represent ESTs specifically regulated by transection and gray bars ESTs regulated by transection and crush). Graph on the right illustrates the time course and number of ESTs up or down-regulated in the retina at each time after IONC (white bars represent ESTs specifically regulated by crush and gray bars ESTs regulated by transection and crush). In C and D the regulated sequences (either genes or ESTs) were clustered according to when they started to show regulation and how long they were regulated either at one time point (12 h, 24 h, etc) or at several (regulated from 12 h to 24 h, from 12 h to day 3, etc). The number of upregulated sequences is shown in the positive y-axis and number of downregulated ones in the negative y-axis. All sequences were significantly regulated (p value FDR <0,01 and a B value >0) compared to naïve expression levels.