Figure 8. Western blotting for the cleaved Notch intracellular domain. A: The results demonstrate reduced levels of Notch intracellular domain (NotchIC) at 24 h after wounding (Wd) and after TPA treatment. Immunofluorescence staining for Ki67 (green) demonstrates the number of proliferating basal cells to be highest in the TPA-treated cornea at 24 h (B) followed by the wounded cornea at 24 h (C) and a normal unwounded cornea (D). The nuclei are stained red with propidium iodide.