Figure 4. Immunohistochemical staining for NotchIC, the cleaved Notch intracellular fragment in developing mouse corneas. A: At P10, weak NotchIC staining is found in occasional basal cell of the corneal epithelium. B: By P15, NotchIC is present in many of the basal cells. (C) At P90, NotchIC is found throughout the basal and immediate suprabasal layers of the mouse corneal epithelium. D: In the limbal epithelium at P90, NotchIC is seen in few cells, mostly in suprabasal cells. The area closest to the central cornea (left side of the tissue) demonstrates more staining. Incidentally, NotchIC staining is also noted in the keratocytes (A-D). Bar=30 μm.