Figure 1. Relative quantitative reverse transcription polymerase chain reaction comparing the expression of Notch pathway genes between the limbal and central human corneal epithelium. All results are after normalization to 18S rRNA. Higher expression in the central cornea is noted for Hes1 (1.4 fold) and Hes5 (1.7 fold). The expression of Notch1 (0.7 fold) and Jagged1 (0.8 fold) was lower in the central cornea compared to the limbus. The expression of Jagged2, Notch2, and Delta1 appeared slightly lower in the central cornea, but this did not reach statistical significance (*p<0.05). As a positive control, the expression of limbal markers, DeltaNp63 and ABCG2, was markedly higher in the limbus than the central cornea. Representative gels of the relative quantitative RT–PCR for Hes1 and Hes5 are shown.