Figure 4. Western blot analysis using monoclonal antibodies against copine1 and β-actin on the traditional one-dimensional SDS–PAGE of trabecular meshwork cell total proteins. Expressions of untreated and A23187-treated copine1 were analyzed using the western blot method. The expression of β-actin was used as a control. The expression levels were quantitated by measuring band intensities and were expressed as fold induction of that in controls. NTM: normal TM cells; GTM: glaucomatous TM cells. Data are means±SEM of three independent experiments. The asterisk indicates a p<0.01 versus untreated NTM; the double asterisk indicates a p<0.01 versus untreated GTM; and the sharp (hash mark) indicates a p<0.01 versus NTM treated with A23187.