Figure 2. PDGF-mediated STAT3 induction in human corneal fibroblasts involves JAK2-STAT3 pathway. Human corneal fibroblasts were induced with the mixture of PDGF (AA, AB, and BB) at a final concentration of 20 ng/ml for 1 h or 8 h in the presence or absence of 100 μM AG-490. Protein levels were measured by immunoblotting using a polyclonal STAT3 antibody. PDGF treatment significantly increased STAT3 protein expression by 2.5 fold (1 h) to 2.96 fold (8 h). β-Actin immunoblot shows the equal loading in each lane.