Figure 3. PKC inhibitor perturbs MIP/AQP0 membrane localization without affecting AQP1 membrane localization in differentiating lens explants. Images are shown of MIP/AQP0 immunofluorescence (red; A) and AQP1 immunofluorescence (green; B) in rat lens epithelial explants cultured in 100 ng/ml FGF-2 and 0.2% DMSO for 72 h (control, as described in Methods). Images are shown of MIP/AQP0 immunofluorescence (red; D) and AQP1 immunofluorescence (green; E) in rat lens epithelial explants cultured in 100 ng/ml FGF-2 in the presence of 4 μM Go6976 plus 0.2% DMSO for 72 h (as described in Methods). C and F show the merged images of MIP/AQP0 immunofluorescence and AQP1 immunofluorescence and the DAPI nuclear staining in controls (A and B) and Go6976 experiments (D and E), respectively. Scale bars represent 20 μm.