Figure 1. PKC inhibitor does not affect MIP transcription in rat lens explants. Real-time PCR analysis is shown of Mip mRNA from rat lens epithelia explants cultured for 72 h with 100 ng/ml FGF-2 in the absence (red) or presence of Go6976 (4 μM; blue hatched) as indicated in Methods. Standard deviations are indicated in green. The endogenous Mip mRNA level is not significantly affected in rat lens explants treated with PKC inhibitor, Go6976.