Figure 8. The effect of TGZ on TGFβ2-induced p38 activation.A: ARPE-19 cells were treated with TGFβ2 alone or were pretreated with 10 μM TGZ for 1 h and then stimulated with TGFβ2 for the indicated time periods. Cells were harvested and subjected to western blot analysis with phosphospecific antibodies of p38 MAPK. Equal protein loading was confirmed by the reprobing of membranes with total p38 MAPK antibody. B: After densitometric scans of triplicate blots, values for p-p38 MAPK (after the induction for 6 h) were normalized to total p38 MAPK. *p<0.05 versus untreated cells. C: ARPE-19 cells were treated with TGFβ2 alone or were pretreated for 1 h with the SB203580 or TGZ at the indicated concentrations and then stimulated with TGFβ2 for an additional 6 h. Cells were harvested and subjected to western blot analysis with antibodies against phosphorylated forms of p38 MAPK and HSP27. Loading equality was confirmed with antibodies against total p38 MAPK. D: After densitometric scans of triplicate blots, values for p-HSP27 were normalized to total p38 MAPK. *p<0.05 versus untreated cells. ^#p<0.05 versus TGFβ2-treated cells.