Figure 7. The TGZ inhibition effect is independent to PPARγ activation. A: ARPE-19 cells were pretreated with different doses of GW9662 (5–20 μM, 2 h) before TGZ combing with TGFβ2 treatment for additional 48 h. Immunoblot results are from a representative experiment with β-actin as loading control. B: ARPE-19 cell monolayers were either treated with TGFβ2 or pretreated with 10 μM GW9662 before TGZ combing with TGFβ2 treatment and wounded with a P-200 pipette tip immediately. Wells were photographed at 48 h adjacent to a reference line scraped on the bottom of the plate.