Figure 5. TGZ reduces TGFβ2-promoted healing of wounds in ARPE-19 cell monlayers.Cell monolayers were pretreated with vehicle (DMSO), TGZ (1, 5, 10 μM), or 10 μM SB203580 for 1 h and then treated with 4 ng/ml TGFβ2 and wounded with a P-200 pipette tip immediately after 0 h. Wells were photographed at 0, 24, and 48 h adjacent to a reference line scraped on the bottom of the plate. A: Panels show migration of cells pretreated with either DMSO, 10 μM TGZ, or 10 μM SB202190 before treatment with TGF-β2 for 0, 24, and 48 h as indicated. B: Percent of remaining wound width in monolayer cell sheet. The data show the percent remaining wound closure in each culture condition. For this value, the width was measured at three different locations in the wound and the mean value was compared to the width of the original closure (0 h). All experiments were in triplicate. Bars represent the mean ± SD ^#p<0.01 versus 24 h untreated cells and *p<0.05 versus 24 h TGFβ2-treated cells. **p<0.05 versus 48 h TGFβ2-treated cells.