Figure 2. Fetal γ-crystallin binding to old α-crystallins. Elution profiles of γ-crystallins in the full (top) versus empty (bottom) chambers of microequilibrium dialysis of old α-crystallins and fetal calf γ-crystallins. See Methods for details of C₁₈ reverse phase chromatography to resolve γ-crystallin peaks. Numbered peaks were quantitated and used to compute binding ratios listed in Table 1.