Figure 1. Old γ-crystallins binding to old α-crystallins. Elution profiles are shown of γ-crystallins in the full (top) versus empty (bottom) chambers of microequilibrium dialysis of old α-crystallins and old γ-crystallins. See Methods for details of C₁₈ reverse phase chromatography to resolve γ-crystallin peaks. Numbered peaks were quantitated and used to compute binding ratios listed in Table 1.