Figure 7. Enhanced green fluorescent protein and proliferating cell nuclear antigen expression in Tg(1016α1tubulin:EGFP) and Tg(ccnb1:EGFP)^nt18 72 hpf larva head sections. Enhanced green fluorescent protein (EGFP; C, F), proliferating cell nuclear antigen (PCNA; B, E), and merged expression patterns (A, D) are shown in 72 hpf larval head sections. In the Tg(1016α1tubulin:EGFP) zebrafish, EGFP was predominantly expressed throughout the retina, while PCNA was expressed in the retinal margin, mandibular tissue, the dorsal epithelium, and tectum (A-C). In the Tg(ccnb1:EGFP)^nt18 zebrafish, EGFP was restricted to the retinal margins similar to PCNA, but EGFP was also expressed in other head tissues that coexpressed PCNA (D-F).