Figure 6. Enhanced green fluorescent protein expression during retinal development in Tg(1016α1tubulin:EGFP) zebrafish. Enhanced green fluorescent protein (EGFP; B, E, H), proliferating cell nuclear antigen (PCNA; C, F, I), and merged expression patterns (A, D, G) are shown at 54 hpf, 72 hpf, and 96 hpf of retinal development. At 54 hpf, EGFP is expressed throughout the entire retina, while PCNA expression is lower in the central retina relative to the retinal margin (A-C). At 72 hpf, EGFP continues to be expressed throughout the central retina, while PCNA expression is largely absent in the central retina and highly expressed in the cells near the margin (D-F). By 96 hpf, EGFP expression decreases in the central retina but remains in the margin, while PCNA expression remains restricted to the retinal margin (G-I).