Figure 1. Diagram of the Tg(ccnb1:EGFP)^nt18 expression vector. The pT2KXIG plasmid containing the 1.5 kb cyclin B1 promoter (light blue) was cloned upstream of the enhanced green fluorescent protein (EGFP) open reading frame (ORF; green), which was located within the Tol2 transposable element (yellow) in the pT2KXIG plasmid. The SV40 transcriptional termination and polyA signal (pink) follows downstream of the open reading frame.