Figure 2. Examples of karyotypes of cultured human corneal endothelial cells. A:Normal karyotype (46, XX) was observed in passage 5 of 60-year-old donor (#6). B: Sex chromosome Y deficiency (45, X, –Y) was observed in passage 5 of 61-year-old donor (#7). A circle shows the deficiency of sex chromosome Y. C: Chromosome 8 trisomy (47, XX, +8) was observed in passage 3 of 38-year-old donor (#4). A circle shows chromosome 8 trisomy.