Figure 9. Exogenous human holo-transferrin does not protect Müller glial cells from wild-type mice from iron-induced stress. A,B: The number of Müller glial (MG) cells from wild-type (WT) mice in culture at the first passage after treatment with 100 µM of FeCl₃-NTA (FN), during 96 h was evaluated by counting in comparison to control condition A: The MG cells from WT mice at the first passage were treated with different concentrations (2.5, 5, 10, and 20 ng/ml) of human holo-transferrin (h holo-Tf). Their number was determined by counting in comparison to control condition. Each column represents the mean ±SEM. The asterisk represents statistical significance of differences for treated by h holo-Tf alone in relation respectively to control and to treated by FN alone, p<0.05 Data considered not significant compared to control or to treated by FN alone is marked NS. B: The MG cells from WT mice at the first passage were treated with 100 µM of FN in presence of different concentrations (2.5, 5, 10, and 20 ng/ml) of h holo-Tf in the medium. Their number was determined by counting in comparison to control condition. Each column represents the mean ±SEM. The asterisk represents statistical significance of differences for treated by h holo-Tf after FN in relation, respectively, to control and to treated by FN alone, p<0.05. Data considered not significant compared to control or to treated by FN alone is marked NS.