Figure 7. Mouse transferrin and human transferrin mRNA expression are modified after iron stress. A: Mouse transferrin (mTf) mRNA expression was quantified by RT-qPCR in Müller glial (MG) cells respectively from wild-type (WT) and transgenic (Tg) mice in control condition and after addition of 100 µM FeCl₃-NTA (FN) in the medium during 96 h. Each column represents the mean ± SEM. The double asterisk represents statistical significance of differences between treated and control, respectively, for WT and Tg, and between control and treated, p<0.01. B: Human transferrin (hTf) mRNA expression was quantified by RT-qPCR in MG cells, respectively, from Tg mice in control condition and after addition of 100 µM FN in the medium during 96 h. Each column represents the mean ±SEM. The double asterisk represents statistical significance of differences from control, p<0.01 In both figures, data considered not significant is marked NS.