Figure 6. Mouse transferrin and human transferrin proteins expression are modulated after iron stress. A: Mouse transferrin (mTf) secretion was measured by radioimmunoassay (RIA) in the culture medium of Müller glial (MG) cells from wild-type (WT) and transgenic (Tg) mice in control condition or after addition of 100 µM of FeCl₃-NTA (FN) during 96 h. Each column represents the mean ±SEM. The double asterisk represents statistical significance of differences between treated and control, respectively, for WT and Tg, p<0.01. B: Human transferrin (hTf) secreted was quantified by ELISA assay in the culture medium of MG cells from Tg mice in control condition or after addition of 100 µM of FN during 96 h. Each column represents the mean ±SEM. The double asterisk represents statistical significance of differences from control, p<0.01 In both figures, data considered not significant is marked NS.