Figure 5. Müller glial cells from transgenic mice are more resistant to iron-mediated stress than Müller glial cells from wild-type mice. A: Culture of Müller glial (MG) cells from wild-type (WT) and transgenic (Tg) mice at the first passage were treated with 100 µM of FeCl₃-NTA (FN), during 96 h. The number of cells was evaluated by counting in comparison to control condition. Each column represents the mean ±SEM. The triple asterisk represent statistical significance of differences between treated and control, respectively, for WT and Tg, and between control and treated, p<0.001. B: Release of lactate dehydrogenase (LDH) was measured after incubation of MG cells with 100 µM of FN. The percent (%) release of LDH from MG cells was reported to the total LDH release. Each column represents the mean ±SEM. The double asterisk represents statistical significance of differences from control, p<0.01 In both figures, data considered not significant is marked NS.