Figure 4. Transferrin secretion in medium of Müller glial cells from 8 to 12-day-old wild-type and transgenic mice decreases with the subcultures. A: Mouse transferrin (mTf) secretion in medium of cultured Müller glial (MG) cells from wild-type ( WT) and transgenic (Tg) mice at confluency after primary culture (P0) and at the first (P1) and the second passages (P2) was measured by radioimmunoassay (RIA; ng/ml). Each column represents the mean ±SEM. The asterisk represents statistical significance of differences between WT and Tg at P0, p<0.05. The triple asterisk represent statistical significance of differences from WT and Tg mice betwen P1 and P2 compared to P0 and between P1 and P2 themselves, p<0.001. B: Human transferrin (hTf) secretion in medium of cultured MG cells from WT and Tg mice at confluency after primary culture (P0) and at the first (P1) and the second passages (P2) was measured by ELISA assay (ng/ml). Each column represents the mean ±SEM. The asterisk represents statistical significance of differences from P0, p<0.05. The double asterisk represents statistical significance of differences from WT and Tg mice betwen P1 and P2 compared to P0 and between P1 and P2 themselves, p<0.0.1.