Figure 2. Mouse transferrin is synthetized and localized in one-month-old mouse retina. A: Non-immune serum was used as a control. B-C: Mouse transferrin (mTf; green) is localized on frozen sections of wild-type (WT) mice (A,B) and in transgenic (Tg) mice (C). Scale bar equals 100 µM. The following abbreviations are used: Ganglion cell layer (GCL), inner nuclear layer (INL), inner plexifrom layer (IPL), inner segments (IS), outer limiting membrane (OLM), outer nuclear layer (ONL), outer plexifrom layer (OPL), outer segments (OS), retinal capillaries (RC), retinal pigmented epithelium (RPE). D: mTf concentration (ng/ml) was measured by radioimmunoassay (RIA) in supernatant of retinal extracts of WT and Tg mice . Each column represents the mean ±SEM. The double asterisk represents statistical significance of differences from control, p<0.01. E: mTf mRNA expression was quantified by RT-qPCR in retinal extracts from WT and Tg mice. Each column represents the mean ±SEM. The asterisk represents statistical significance of differences from control, p<0.05.