The distribution of water along the sagittal axis in fixed and unfixed human lenses. Comparison of the distributions of water
along the sagittal axis in an unfixed seven-year-old lens (green diamond), which was calculated from the RI gradient obtained
with MRI [12
], and in a fixed eight-year-old lens (red diamond) as determined by the use of Raman microspectroscopy [5
] is shown. The dimensions were normalized to eliminate differences in the thickness of the lenses due to possible shrinkage
of the fixed lens.